nf-κb inhibitor ppm-18  (ApexBio)

Journal: Calcified Tissue International

Article Title: Oridonin Attenuates Thioacetamide-Induced Osteoclastogenesis Through MAPK/NF-κB Pathway and Thioacetamide-Inhibited Osteoblastogenesis Through BMP-2/RUNX2 Pathway

doi: 10.1007/s00223-023-01080-5

Figure Lengend Snippet: ORI inhibits TAA-induced activation of MAPK/NF-κB pathway. A, E Representative western blot images of the effects of TAA and ORI on MAPK/NF-κB pathway. B–D, F–H The band intensity ratios relative to TLR4(relatived to β-actin), p-p65, p-IκBα, p-ERK, p-p38 and p-JNK were quantified. Values were expressed as mean ± SD of three individual experiments; *** P < 0.001

Article Snippet: The p38 MAPK inhibitor (SB202190) and NF-κB inhibitor (PPM-18) were supplied by APExBIO.

Techniques: Activation Assay, Western Blot

Journal: Calcified Tissue International

Article Title: Oridonin Attenuates Thioacetamide-Induced Osteoclastogenesis Through MAPK/NF-κB Pathway and Thioacetamide-Inhibited Osteoblastogenesis Through BMP-2/RUNX2 Pathway

doi: 10.1007/s00223-023-01080-5

Figure Lengend Snippet: TAA promotes osteoclastogenesis by activating MAPK/NF-κB pathway, while ORI can inhibit this effect. A, C Osteoclast differentiation was performed after cells were treated with NF-κB inhibitor (PPM-18) or p38 MAPK inhibitor (SB202190) for 2 h. Representative western blot images of the effects of TAA and ORI on osteoclast differentiation. B, D The band intensity ratio of c-Fos, NFATc1, TRAP and cathepsin K relative to β-actin were quantified. Values were expressed as mean ± SD of three individual experiments; * P <0.05, ** P < 0.01, *** P < 0.001

Article Snippet: The p38 MAPK inhibitor (SB202190) and NF-κB inhibitor (PPM-18) were supplied by APExBIO.

Techniques: Western Blot